
The American veteran said she still hoped to play at the Adelaide International later this month ahead of the Australian Open, the first Grand Slam of the year.
“Unfortunately I will not be starting my season in Brisbane due to an unexpected setback during my training,” the 39-year-old seven-time major winner said in a statement.
“I look forward to being in Australia in the new year and will see everyone in Adelaide.”
The tournament, featuring a strong field including world number one Ashleigh Barty and Australian Open champion Naomi Osaka, gets underway on January 6.
Organisers will announce a replacement for Williams on Thursday.