
Ohashi produced a strong finishing freestyle leg to touch the wall in 2:08.52s, with Alex Walsh second in 2:08.65s and fellow American Kate Douglass third in 2:09.04s.
Defending champion and world record-holder Katinka Hosszu came seventh.
After winning the 400m medley Ohashi credited a wave of hometown support in helping her realise her dream.
And she tapped into it again today.
She was second behind earlier pace setter Yu Yiting after the butterfly and backstroke legs, but timed her race perfectly with a powerful breaststroke swim before bringing it home in style.
She has now dethroned veteran Hosszu twice, with the Hungarian virtually untouchable in this event since winning the Rio Olympics, claiming gold at the 2017 and 2019 world championships.
But the writing was on the wall when she only qualified seventh-fastest after finishing fifth in the 400m medley.